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. 2022 May 18;10:849854. doi: 10.3389/fcell.2022.849854

FIGURE 3.

FIGURE 3

Edaravone downregulates ACSL4 at 2 days post SCI. (A) Upper, schematic diagram of ACSL4 detected injured epicenter and caudal spinal cord ventral horn (3 mm to the lesion) at 2 days post SCI. Lower, immunofluorescence images of ACSL4 (green) in the lesion epicenter and caudal spinal cord section. Nuclei were stained with DAPI (blue). Scale bar,100 μm. (B) Quantification of the ACSL4 intensity in the lesion and caudal segment. (C,D) Representative immunofluorescence images of of ACSL4 (green)/NeuN (red) (C) and ACSL4 (green)/ChAT (red) (D) in the caudal spinal cord at 3.0 mm to the lesion at 2 days post SCI. Scale bar,100 μm. (E,F) Quantification of neuronal (NeuN positive) ACSL4 (E) and motor neuronal (ChAT positive) ACSL4 (F). Data are expressed as mean ± standard deviation. Statistical significance was determined by two-way ANOVA with Sidak’s multiple comparisons test (B) or one-way ANOVA with Tukey’s post hoc test (E,F). *p < 0.05, **p < 0.01, and ***p < 0.001, n = 3, bar = 100 μm.