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. 2022 May 18;10:849854. doi: 10.3389/fcell.2022.849854

FIGURE 4.

FIGURE 4

Edaravone downregulates 5-LOX at 2 days post SCI. (A) Upper, schematic diagram of 5-LOX detected injured epicenter and caudal spinal cord ventral horn (3 mm to the lesion) at 2 days post SCI, Lower, immunofluorescence images of 5-LOX (green) in the lesion epicenter and caudal spinal cord section. Nuclei were counterstained with DAPI (blue). Scale bar,100 μm. (B) Quantification of the 5-LOX intensity in the lesion and caudal segment. (C,D) Representative immunofluorescence images of 5-LOX (green)/NeuN (red) (C) and 5-LOX (green)/ChAT (red) (D) in the caudal spinal cord at 3.0 mm to the lesion at 2 days post SCI. Scale bar, 100 μm. (E,F) Quantification of neuronal (NeuN positive) 5-LOX (E) and motor neuronal (ChAT positive) 5-LOX (F). Nuclei were stained using DAPI in blue. Data are expressed as mean ± standard deviation. Statistical significance was determined by two-way ANOVA with Sidak’s multiple comparisons test (B) or one-way ANOVA with Tukey’s post hoc test (E,F). *p < 0.05, **p < 0.01, and***p < 0.001, n = 3, bar = 100 μm.