Figure 5.

(a) Gene structures and single-nucleotide polymorphisms (SNPs) of GmRSS3L and GsRSS3L; (b) predicted three-dimensional structures of protein GmRSS3L and GsRSS3L, as well as the α-helix region that may be affected by the two nonsynonymous mutants, as indicated by the red box; (c) qRT-PCR quantification of SMV genomic RNA in Nicotiana benthamiana leaves with transient expression of SMV infectious clone with GmRSS3L, GsRSS3L and empty vector of pCambia3301 as control, at 3, 5, 7 days post-inoculation (dpi). The * indicates a significant difference at p < 0.05.