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. 2022 Jul 11;23(14):7659. doi: 10.3390/ijms23147659

Figure 2.

Figure 2

Calcitriol and curcumin inhibited endothelium-induced TNBC-VM capacity in co-cultures. EA.hy926-induced VM was observed in co-cultures of HCC1806 (A) and MBCDF-T (B) cells incubated with vehicle (VH, ethanol). The presence of calcitriol (CAL, 10 nM) changed cell morphology from spindle-shape to cobblestone-like and abrogated VM formation, while curcumin (CUR, 20 µM) affected the 3D VM structure. The combination of calcitriol with curcumin (C+C) further inhibited VM in both TNBC cells. The number of meshes and segments per visual field were counted by three different observers in hot spots in at least 10 different photographs, considering 3 different experiments for each cell line. The graphics depict the average number of meshes and segments per visual field. * p < 0.001 vs. VH. ** p < 0.01 vs. C+C. Magnification is 10×.