Figure 3.

In MBCDF-T/EA.hy926 co-cultures, calcitriol and curcumin inhibited the phosphorylation of several protein targets of the MAPK, PI3K/Akt/mTOR, PI3K/Akt/CREB and PI3K/Akt/Gsk signaling pathways. The phosphorylation profile of kinases involved in signaling pathways relevant to VM was differentially inhibited by calcitriol (CAL, 10 nM), curcumin (CUR, 20 µM) and their combination (C+C). A semi-supervised hierarchical clustering of the targets differentially phosphorylated detected with a multiplex antibody array was carried out and results are shown in the heat map clustered in three main signaling pathways: PI3K/Akt, mTOR and MAPK. The more strongly phosphorylated kinases in vehicle-treated co-cultures (VH) are labeled in black. Kinases mostly inhibited by calcitriol are labeled in red, while phosphorylated protein targets more strongly downregulated by curcumin are labeled in blue. N = 4.