Table 2.
(a) Preparation of the trickling solution of different concentrations; (b) preparation of the trickling solution from stock solution; and (c) concentration of trickling solutions in comparison with previous studies.
(a) | ||||||
---|---|---|---|---|---|---|
Preparation of trickling solution directly from different compounds | 250 mM solution | 500 mM solution | 750 mM solution | |||
Lithium chloride monohydrate (LiCl × H2O) |
15.10 g L−1 | 30.20 g L−1 | 45.29 g L−1 | |||
Lithium chloride anhydrate (LiCl) |
10.60 g L−1 | 21.20 g L−1 | 31.79 g L−1 | |||
(b) | ||||||
Preparation of stock solution | Preparation of trickling solution from the stock | |||||
agent used (g) | final volume (mL) | final concentration (M) | stock solution (mL) | final volume (mL) | final concentration (mM) | |
LiCl anhydrate | 500 | 2137 | 5.5 | 45.3 | 1000 | 250 |
LiCl monohydrate | 500 | 1500 | 5.5 | 45.3 | 1000 | 250 |
(c) | ||||||
Concentration | Single volume/colony |
Way of administration | Single dose/ colony Li+ basis (mg) |
Reference | ||
Lithium chloride | 25 mM | ad libitum | feeding sugar syrup |
- | Ziegelmann et al., 2018 [22] Ziegelmann et al., 2019 [23] |
|
Lithium chloride | 50 mM | ad libitum | feeding sugar syrup |
- | Ziegelmann et al., 2018 [22] | |
Lithium chloride | 50 mM | ad libitum | feeding sugar dough | - | Ziegelmann et al., 2019 [23] | |
Lithium chloride | 25 mM | 1000 mL | feeding sugar syrup |
173.5 | Kolics et al., 2019 [30] | |
Presern et al., 2020 [25], Kolics et al., 2021a [31] | ||||||
Lithium chloride | 250 mM | 40 mL | trickling | 69.4 | Kolics et al., 2021b [31], Kolics et al., 2020 [32], present study |
|
Lithium chloride | 500 mM | 40 mL | trickling | 138.8 | present study | |
Lithium chloride | 750 mM | 40 mL | trickling | 208.2 | present study | |
Lithium citrate | 5 mM | 1000 mL | feeding sugar syrup |
101 | Stanimirovic et al., 2022 [29] | |
Lithium citrate | 10 mM | 1000 mL | feeding sugar syrup |
202 | Stanimirovic et al., 2022 [29] | |
Lithium citrate | 15 mM | 1000 mL | feeding sugar syrup |
302.9 | Stanimirovic et al., 2022 [29] | |
Lithium citrate | 20 mM | 1000 mL | feeding sugar syrup |
403.9 | Stanimirovic et al., 2022 [29] | |
Lithium citrate | 25 mM | 1000 mL | feeding sugar syrup |
504.9 | Stanimirovic et al., 2022 [29] |