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. 2022 Jul 15;13(7):633. doi: 10.3390/insects13070633

Table 2.

(a) Preparation of the trickling solution of different concentrations; (b) preparation of the trickling solution from stock solution; and (c) concentration of trickling solutions in comparison with previous studies.

(a)
Preparation of trickling solution directly from different compounds 250 mM solution 500 mM solution 750 mM solution
Lithium chloride monohydrate
(LiCl × H2O)
15.10 g L−1 30.20 g L−1 45.29 g L−1
Lithium chloride anhydrate
(LiCl)
10.60 g L−1 21.20 g L−1 31.79 g L−1
(b)
Preparation of stock solution Preparation of trickling solution from the stock
agent used (g) final volume (mL) final concentration (M) stock solution (mL) final volume (mL) final concentration (mM)
LiCl anhydrate 500 2137 5.5 45.3 1000 250
LiCl monohydrate 500 1500 5.5 45.3 1000 250
(c)
Concentration Single
volume/colony
Way of administration Single
dose/
colony
Li+ basis (mg)
Reference
Lithium chloride 25 mM ad libitum feeding
sugar syrup
- Ziegelmann et al., 2018 [22]
Ziegelmann et al., 2019 [23]
Lithium chloride 50 mM ad libitum feeding
sugar syrup
- Ziegelmann et al., 2018 [22]
Lithium chloride 50 mM ad libitum feeding sugar dough - Ziegelmann et al., 2019 [23]
Lithium chloride 25 mM 1000 mL feeding
sugar syrup
173.5 Kolics et al., 2019 [30]
Presern et al., 2020 [25],
Kolics et al., 2021a [31]
Lithium chloride 250 mM 40 mL trickling 69.4 Kolics et al., 2021b [31],
Kolics et al., 2020 [32],
present study
Lithium chloride 500 mM 40 mL trickling 138.8 present study
Lithium chloride 750 mM 40 mL trickling 208.2 present study
Lithium citrate 5 mM 1000 mL feeding
sugar syrup
101 Stanimirovic et al., 2022 [29]
Lithium citrate 10 mM 1000 mL feeding
sugar syrup
202 Stanimirovic et al., 2022 [29]
Lithium citrate 15 mM 1000 mL feeding
sugar syrup
302.9 Stanimirovic et al., 2022 [29]
Lithium citrate 20 mM 1000 mL feeding
sugar syrup
403.9 Stanimirovic et al., 2022 [29]
Lithium citrate 25 mM 1000 mL feeding
sugar syrup
504.9 Stanimirovic et al., 2022 [29]