Figure 5.

TLR1-TIR and TLR2-TIR homodimerization. (A) A conserved cysteine, C707 (red), within the crystallographic homodimer in TLR1 is shown (PDB accession 1fyv). (B) The cysteine is partially buried by the conserved BB-loop and is conserved within multiple TLR TIR domains and the IL-1R8-TIR domain. (C) Analytical Superose-12 of recombinant TLR1-TIR in 1 mM DTT (top), incubated for 30 min in 50 mM DTT (middle), and the TLR1-TIR C707A mutant in 1 mM DTT (bottom). (D) Analytical Superose-12 of recombinant TLR2-TIR in 1 mM DTT (top), incubated for 30 min in 50 mM DTT (middle), and the TLR2-TIR C713A mutant in 1 mM DTT (bottom). Buffer for analytical sizing was 50 mM Na₂HPO₄, pH 6.5, 150 mM NaCl, 1 mM DTT.