Figure 8.

Liver biopsies from Control Pony 1 (A,C,E,G,I) and Vaccine Pony 1 (B,D,F,H,J) revealed a mild, periportal inflammatory cell infiltration in haematoxylin and eosin-stained samples (A,B). The infiltrate was composed of a few macrophages (arrows, (C,D)) and T lymphocytes (arrows, (E,F)). B lymphocytes were rarely observed (arrow, (G,H)). EqHV nucleic acid was distributed multifocally within the cytoplasm of hepatocytes, as demonstrated by fluorescent in situ hybridization/FISH (I,J). Bar = 50 µm (A–H), 25 µm (I,J).