Table 3.
Equine hepacivirus (EqHV) quantitative polymerase chain reaction (qPCR) results from liver biopsies of six experimental ponies after EqHV infection (n = 6 on day +13; n = 6 on day +97), as well as the corresponding serum EqHV qPCR results (n = 6 on day +14; n = 6 on day +98) of these ponies. All ribonucleic acid (RNA) samples from liver biopsies were analysed in duplicate, except for the biopsy from Vaccine Pony 3 on day +97, for which only a single analysis was done c. The mean viral load of the two sample duplicates was determined for each liver biopsy. Mean liver viral load values that were below the lower limit of quantitation (LLOQ = 1 × 103 viral copies/50 ng total RNA) of this qPCR assay are indicated as <LLOQ. Serum viral load values that were below the lower limit of quantitation (LLOQ = 7.2 × 104 viral copies/mL of serum) of this qPCR assay are indicated as <LLOQ.
| Mean Liver Viral Load (Viral Copies/50 ng Total RNA; LLOQ = 1 × 103 Copies/50 ng Total RNA) |
Serum Viral Load (Viral Copies/mL Serum; LLOQ = 7.2 × 104 Copies/mL Serum) |
|
|---|---|---|
| Day +13 | Day +14 | |
| Vaccine Pony 1 | 8.72 × 104 | 9.51 × 106 |
| Vaccine Pony 2 | 3.55 × 104 | 1.14 × 107 |
| Vaccine Pony 3 | 7.52 × 104 | 1.36 × 107 |
| Vaccine Pony 4 | 1.30 × 105 | 1.47 × 107 |
| Control Pony 1 | 8.77 × 104 | 1.50 × 107 |
| Control Pony 2 | 7.66 × 104 | 9.80 × 106 |
| Day +97 | Day +98 | |
| Vaccine Pony 1 | 1.27 × 103 | <LLOQ |
| Vaccine Pony 2 | <LLOQ | <LLOQ |
| Vaccine Pony 3 | <LLOQ c | <LLOQ |
| Vaccine Pony 4 | 1.00 × 104 | 4.66 × 105 |
| Control Pony 1 | 9.28 × 103 | 4.13 × 106 |
| Control Pony 2 | <LLOQ | 3.01 × 105 |