Table 1.
Primary and secondary antibodies used for Western blotting.
| Primary Antibody | Dilution | Catalogue No. | Company | Secondary Antibody | Dilution | Catalogue No. | Company |
|---|---|---|---|---|---|---|---|
| AMPKα | 1:1000 | sc-5298 | Santa | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
| C/EBPα | 1:1000 | sc-365318 | Santa | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
| HSL | 1:500 | ab45422 | Abcam | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
| IL-1β | 1:1000 | 12242s | Cell Signaling | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
| MCP1 | 1:1000 | ab25124 | Abcam | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
| PPARγ | 1:500 | 2430s | Cell Signaling | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
| pAMPKα | 1:500 | 2535s | Cell Signaling | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
| pHSL | 1:500 | 4139s | Cell Signaling | Goat-anti-rabbit | 1:5000 | ADI-SAB-300 | Enzo |
| TNFα | 1:1000 | sc-52746 | Santa | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
| GAPDH | 1:1000 | Sc-32233 | Santa | Goat-anti-mouse | 1:5000 | ADI-SAB-100 | Enzo |
AMPKα, adenosine 5-monophosphate-activated protein kinase alpha; C/EBPα, cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT) enhancer-binding protein alpha; HSL, hormone-sensitive lipase; IL-1β, interleukin-1 beta; MCP1, monocyte chemoattractant protein 1; PPARγ, peroxisome proliferator-activated receptor γ; pAMPKα, phospho-AMPKα; pHSL, phospho-HSL; TNFα, tumor necrosis factor alpha; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Santa Cruz Biotechnology, CA, USA; Cell Signaling Technology Inc., Danvers, MA, USA; Abcam, Cambridge, UK; Enzo Life Science, Farmingdale, NY, USA.