Figure 1.

Doubling times of iPSCs vary little during culturing. (A) WTC-11 cell line. (B) Tuba1-GFP cell line. Cell counts were obtained with each passage using an automated cell counter, and the time between each passage was recorded in order to calculate population doubling times. Passage numbers reflect documented passages following the creation of WTC-11 iPSC line and begin at the passage number for which they were thawed for these experiments. There was no significant change in doubling times between the first ten and last ten passages in either cell line (unpaired t-test).