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The presence of an aneuploidy event in the Tuba1-GFP iPSC population is confirmed via chromosome spreads. Each sample reflects the ploidy of the passage population. Passages 22 to 26 indicate a progressive shift from normal ploidy to aneuploidy as the chromosome numbers of the populations shift from 46 to 47. The aneuploidy of the population then persists in the population, as indicated in the passage 40 sample. Each passage reflects >20 cells quantified.
