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. 2022 Jul 4;14(7):1404. doi: 10.3390/pharmaceutics14071404

Figure 6.

Figure 6

In vivo study of DMN for hair regrowth. (A) Physical images of mice treated with RAPA-PLGA and EGCG-KE NP-loaded MNs (H1), RAPA-PLGA-loaded MNs (H2), EGCG-KE NP-loaded MNs (H3), topical RAPA (H4), and topical EGCG (H5). Untreated mice (H0) served as a control. (B) Hair shaft analysis chart of new hair grown on treated mouse dorsal skin as a function of treatment time and day 15 (n = 3, mean ± SD). (C) Hair follicle density analysis chart of new hair grown on treated dorsal skin at day 15 (n = 3, mean ± SD). (D) Western blotting assay of the expression level of proteins related to hair follicle growth activation (β-Catenin, p-AKT). (E) H&E staining images of longitudinal sections (top) and transverse sections (bottom) of mouse dorsal skin (scale bar: 100 μm). A probability value (p < 0.05) by t-test was considered significant (* p < 0.05, *** p < 0.001).