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. 2022 Jun 30;20(7):440. doi: 10.3390/md20070440

Table 2.

Examples of adaptive laboratory evolution reports obtained by different methods. An extended version of this table can be found in the Supplementary Materials (Table S2).

Species Method Target Improvement References
Chlorella sp. 31 cycles under 500 mg/L of phenol Phenol wastewater removal 100% phenol removal in 7 days; maximum biomass concentration increased 2-fold [141]
Chlorella sp. 46 cycles with flue gas Tolerance to flue gas Growth under 10% CO2, 200 ppm NOx, and 100 ppm SOx [78]
Phaeodactylum tricornutum 11 cycles, 5 days each, light-induced oxidative stress supplied by LED Carotenoid content 2-fold higher biomass production and fucoxanthin content [142]
Phaeodactylum tricornutum 35 cycles, 7 days each, of hyposaline treatment Fatty acid content EPA content increased up to 139 µg/mg biomass; improved growth [143]
Picochlorum sp. 390 days under temperature stress Thermotolerance 1.5 °C increase in the maximum tolerable temperature [144]
Tisochrysis lutea 2 rounds of direct evolution + FACS Carotenoid and fatty acid content 3.1-fold fucoxanthin and 1.6-fold DHA higher productivities [29]