Figure 6.

Alcohol-dependent males, but not females, have a basal CRF₁-mediated tone in the central amygdala (CeA). (A) Representative GABA_A-mediated sIPSCs from CeA neurons of dependent female (upper) and male (lower) rats at baseline (left) and during acute application of R121919 (1 µM; right). There was no main effect of Sex or Alcohol Exposure and no interaction effect of R121919 on sIPSC frequency (B), amplitude (C), rise time (D), or decay time (E). Changes from baseline sIPSC properties were assessed using one-sample t-tests where # denotes p < 0.05. Group differences in the sIPSC responses to R121919 were assessed using two-way ANOVA test but were not observed. n = 9–10 neurons per group; N = 5–6 animals per group; bars represent Mean ± SEM.