Figure 1.

Target Enrichment Approaches for NGS. The underlying principles of the two prominent target enrichment technologies are depicted schematically. (A) The PCR amplicon-based method employs PCR amplification using sequence-specific, target-spanning primers to amplify and enrich the genomic regions of interest (targets). Postamplification, in a ligation step, sequencing adapters and barcode indexes are included to generate the genomic library for sequencing. (B) In the hybrid capture method, the input genomic DNA is fragmented using enzymatic or acoustic methods followed by ligation of adapters and barcode indices to generate a genomic library. This is subjected to hybridization using biotin-labeled target sequence-specific probes followed by capture of the hybridized probes using Streptavidin magnetic beads to isolate genomic regions of interest for sequencing.