Figure 1.

Body temperature of cattle immunized with inactivated LSDV vaccine candidates with different amounts of antigen. Body temperature was measured daily from −4 dpv until 28 dpc. Animals were inoculated with inactivated LSDV vaccine candidate with different amounts of antigen and then challenged with virulent LSDV-Macedonia2016. (A) Vaccine virus titer before inactivation was 10⁶ CCID₅₀/mL on BHK-21 cell line. (B,C) Antigen was diluted 1:10 and 1:100, respectively, before addition of adjuvant. (D) Cattle in group D were housed as challenge control group and received PBS instead of vaccine. Body temperature >39.5 °C was defined as increased, and body temperature >40.0 °C indicated fever.