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Determining optimal conditions in terms of viability for steatosis induction in primary broiler hepatocytes incubated with fat emulsion. The first downward arrow (0 h) indicates where culture medium was replaced with steatosis induction medium containing 2.5, 5, 10, or 20% fat emulsion. The second downward arrow at 48 h indicates where the medium in the fat emulsion group was replaced with fresh induction medium. Viability was determined by CCK-8 assay at 0, 6, 12, 24, 48, and 72 h.
