Figure 2.

Effect of fat emulsion on mitochondrial membrane potential and apoptosis of hepatocytes. (a) Representative images of JC-1 staining in different groups (200×). Red fluorescence represents the mitochondrial aggregated form of JC-1, indicating normal membrane potential from intact mitochondria. Green fluorescence represents the monomeric form of JC-1, indicating loss of membrane potential (ΔΨm). (b) The ratio of green to red reflects the percentage of cells in apoptosis. Note: Data are presented as mean ± SEM; statistical differences were assessed by one-way ANOVA with subsequent Tukey’s HSD test (the same below).