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. 2022 Jul 18;11(14):2227. doi: 10.3390/cells11142227

Figure 3.

Figure 3

VMP1 regulates the proliferation of goat myoblasts: (A,B) RT-qPCR was used to determine VMP1 expression levels in myoblasts transfected with pcDNA3.1-VMP1 and siRNA-VMP1. (C) Proliferation of goat myoblasts overexpressing VMP1, measured using a Cell Counting Kit-8. (D) Proliferation of goat myoblasts transfected with siRNA-VMP1. (E) Expression of the cell-proliferation-related genes CDK4, cyclin D1, and cyclin D2 in cells overexpressing VMP1 was determined by RT-qPCR. (F) Expression of cell-proliferation-related genes in cells transfected with siRNA-VMP1. (G) EdU staining of myoblasts overexpressing VMP1, and in which VMP1 is silenced. (H,I) Fold change in the proliferation of myoblasts overexpressing VMP1, and in which VMP1 is silenced. Replicates = 3. Data are shown as the mean ± SE. * p < 0.05 and ** p < 0.01. NC, negative control.