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. 2022 Jul 18;11(14):2227. doi: 10.3390/cells11142227

Figure 7.

Figure 7

miR-124a regulates apoptosis of goat myoblasts: (A,B) Expression levels of cell-apoptosis-related genes (caspase-3, caspase-9, and beclin-1) in cells overexpressing and underexpressing miR-124a were quantified by RT-qPCR. (C,D) Western blot analysis revealed the expression of caspase-3 and beclin-1 after gain and loss of miR-124a. GAPDH was used as an internal reference. (E) Myoblasts overexpressing miR-124a were stained with annexin V–FITC/propidium iodide (PI), and then subjected to flow cytometry. (F) The percentage of miR-124a-overexpressing myoblasts in apoptosis. (G) Flow cytometry of myoblasts underexpressing miR-124a. (H) Percentage of miR-124a-underexpressing myoblasts in apoptosis. Replicates = 3. Data are presented as the mean ± SE; * p < 0.05 and ** p < 0.01. NC, negative control.