Table 3.
Bile acid analyses within accuracy limits based on relative recovery. NA = not applicable, those BAs are not covered by the specific center.
| Center | Matrix | Conc. Level | # BAs in Method | # BAs in Rel. Recovery Limits |
Bile Acid in Relative Recovery Limits | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CA | CDCA | GCA | TCA | DCA | LCA | α-MCA | β-MCA | ω-MCA | |||||
| Center 1 | MeOH:H2O | High | 9 | 2 | - | - | Yes | Yes | - | - | - | - | - |
| Low | 9 | 2 | - | - | Yes | Yes | - | - | - | - | - | ||
| Human serum | High | 9 | 0 | - | - | - | - | - | - | - | - | - | |
| Low | 9 | 1 | - | - | - | Yes | - | - | - | - | - | ||
| Murine serum | High | 9 | 1 | - | - | - | Yes | - | - | - | - | - | |
| Low | 9 | 2 | - | - | Yes | Yes | - | - | - | - | - | ||
| Center 2 | MeOH:H2O | High | 9 | 5 | Yes | - | Yes | Yes | Yes | - | - | Yes | - |
| Low | 9 | 2 | - | Yes | Yes | - | - | - | - | - | - | ||
| Human serum | High | 9 | 1 | - | Yes | - | - | - | - | - | - | - | |
| Low | 9 | 5 | Yes | - | Yes | Yes | Yes | - | - | Yes | - | ||
| Murine serum | High | 9 | 4 | Yes | - | Yes | Yes | Yes | - | - | - | - | |
| Low | 9 | 3 | Yes | - | - | Yes | Yes | - | - | - | - | ||
| Center 3 | MeOH:H2O | High | 7 | 5 | Yes | - | Yes | Yes | Yes | NA | - | Yes | NA |
| Low | 7 | 5 | Yes | - | Yes | Yes | Yes | NA | Yes | - | NA | ||
| Human serum | High | 7 | 5 | Yes | - | Yes | Yes | Yes | NA | - | Yes | NA | |
| Low | 7 | 5 | Yes | - | Yes | Yes | Yes | NA | Yes | - | NA | ||
| Murine serum | High | 7 | 6 | Yes | - | Yes | Yes | Yes | NA | Yes | Yes | NA | |
| Low | 7 | 6 | Yes | - | Yes | Yes | Yes | NA | Yes | Yes | NA | ||
| Center 4 | MeOH:H2O | High | 7 | 2 | - | - | Yes | Yes | - | - | NA | - | NA |
| Low | 7 | 3 | Yes | - | Yes | Yes | - | - | NA | - | NA | ||
| Human serum | High | 7 | 4 | Yes | - | Yes | Yes | - | Yes | NA | - | NA | |
| Low | 7 | 3 | Yes | - | Yes | Yes | - | - | NA | - | NA | ||
| Murine serum | High | 7 | 3 | Yes | - | Yes | Yes | - | - | NA | - | NA | |
| Low | 7 | 2 | Yes | - | Yes | - | - | - | NA | - | NA | ||
| Center 5 | MeOH:H2O | High | 9 | 4 | - | Yes | - | Yes | - | - | Yes | Yes | - |
| Low | 9 | 6 | Yes | - | Yes | Yes | - | - | Yes | Yes | Yes | ||
| Human serum | High | 9 | 5 | - | Yes | - | Yes | Yes | - | Yes | Yes | - | |
| Low | 9 | 5 | Yes | - | Yes | Yes | - | - | Yes | Yes | - | ||
| Murine serum | High | 9 | 3 | - | - | - | Yes | Yes | - | - | Yes | - | |
| Low | 9 | 4 | Yes | - | Yes | - | - | - | Yes | Yes | - | ||
| Center 6 | MeOH:H2O | High | 9 | 6 | Yes | Yes | Yes | Yes | Yes | - | Yes | - | - |
| Low | 9 | 7 | Yes | Yes | Yes | Yes | Yes | - | Yes | - | Yes | ||
| Human serum | High | 9 | 7 | Yes | Yes | Yes | Yes | Yes | - | Yes | - | Yes | |
| Low | 9 | 7 | Yes | Yes | Yes | Yes | Yes | - | Yes | - | Yes | ||
| Murine serum | High | 9 | 6 | Yes | Yes | Yes | Yes | Yes | - | Yes | - | - | |
| Low | 9 | 6 | Yes | Yes | Yes | Yes | Yes | - | Yes | - | - | ||