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. 2022 Jun 23;12(7):583. doi: 10.3390/metabo12070583

Table 5.

LC-gradient conditions for center 1.

Time
(min)
Flow Rate
(mL/min)
Mobile Phase A
(%)
Analysis 0 0.7 85
9 0.7 60
10 0.7 20
13 0.7 0
Column Regeneration 16 0.7 0
16.1 0.7 85
19 0.7 85