Table 7.
Time (min) |
Flow Rate (mL/min) |
Mobile Phase A (%) |
|
---|---|---|---|
Analysis | 0 | 0.4 | 75 |
2 | 0.4 | 75 | |
3.5 | 0.4 | 73 | |
5.5 | 0.4 | 65 | |
10 | 0.4 | 65 | |
11 | 0.4 | 57 | |
12 | 0.4 | 57 | |
14 | 0.4 | 42 | |
17 | 0.4 | 42 | |
17.5 | 0.4 | 35 | |
18 | 0.4 | 20 | |
19 | 0.4 | 0 | |
20 | 0.4 | 0 | |
23 | 0.4 | 75 | |
Column Regeneration | 25 | 0.4 | 75 |