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. 2022 Jun 23;12(7):583. doi: 10.3390/metabo12070583

Table 9.

LC-gradient conditions for center 5.

Time
(min)
Flow Rate
(mL/min)
Mobile Phase A
(%)
Analysis 0 0.4 80
1 0.4 80
5 0.4 65
14.5 0.4 5
14.6 0.4 0
18.5 0.4 0
18.6 0.4 80
Column Regeneration 20 0.4 80