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. 2022 Jun 23;12(7):583. doi: 10.3390/metabo12070583

Table 10.

LC-gradient conditions for center 6.

Time
(min)
Flow Rate
(mL/min)
Mobile Phase A
(%)
Analysis 0 0.5 65
0.25 0.5 65
0.35 0.5 60
1.9 0.5 55
2.1 0.8 45
3.3 1.0 35
3.5 1.0 0
Column Regeneration 4.0 1.0 0
4.1 0.9 65
5 0.5 65