FIG. 3.

RT-PCR of irradiated and nonirradiated RNAs extracted from C. acetobutylicum DSM792. The upper panel represents the amplification of a 650-bp internal fragment of 16S rRNA which functions as an internal standard to ensure that equal amounts of RNA were used in each RT reaction. The lower panel represents the amplification of a 470-bp internal fragment of mTNF-α. RNA extracted from C. acetobutylicum DSM792 was transformed with pIMP-eglACheo-mTNF-α (first and second lanes), pIMP-recA-mTNF-α (third and fourth lanes), pIMP-recAextraCheo-mTNF-α (fifth and sixth lanes), pIMP-eglA-mTNF-α (eighth and ninth lanes), and pIMP-recAdeletedCheo-mTNF-α (tenth and eleventh lanes). The seventh lane shows a positive control for 16S rRNA (PCR performed on chromosomal DNA from C. acetobutylicum).