TABLE 1.
Shuttle plasmids used in this studya
| Plasmid | Promoter |
|---|---|
| pIMP-eglA-mTNF-α | eglA |
| pIMP-eglACheo-mTNF-α | eglA with incorporated Cheo box |
| pIMP-recA-mTNF-α | recA |
| pIMP-recAextraCheo-mTNF-α | recA with extra Cheo box incorporated |
| pIMP-recAdeletedCheo-mTNF-α | recA with Cheo box deleted |
The eglA signal peptide was used in each case for the secretion of mTNF-α.