TABLE 2.
Sequences of the parental and mutated recA and eglA promoters at the 3′ regiona
| Promoter | Type | Sequence |
|---|---|---|
| recA | Parental | 5′ TTAAGGGACTTTTATTATATTATATTGACAAATTAATAAATTACTATATAATTATATGTATAGAACAAATGTTCGAGAGAAAGGTTGGTGAACCCTTAA 3′ |
| ------------------ | ||
| Extra Cheo | 5′ TTAAGGGACTTTGAACATATGTTCTTGACAAATTAATAAATTACTATATAATTATATGTATAGAACAAATGTTCGAGAGAAAGGTTGGTGAACCCTTAA 3′ | |
| ------------------- | ||
| Deleted Cheo | 5′ TTAAGGGACTTTTTGACAAATTAATAAATTACTATATAATTATATGTATAGAACAAATGTTCGAGAGAAAGGTTGGTGAACCCTTAA 3′ | |
| eglA | Parental | 5′ GGAGGAAAAAACTATCTTTTAAAAGTTTATAGTAAATAAAAAAAAATTATTAATGTAAAAATATACTAAGTATAGAATATTTATAATAGGGGGTATTAAC 3′ |
| ------------------ | ||
| ------------------- | ||
| With Cheo | 5′ GGAGGAAAAAACTATCGAACAAAAGTTCATAGTAAATAAAAAAAAATTATTAATGTAAAAATATACTAAGTATAGAATATTTATAATAGGGGGTATTAAC 3′ |
a
−10 and −35 promoter elements are underlined with solid lines. Shine-Dalgarno sequences are boxed. Sequences to be mutated are underlined with a broken line. Cheo boxes are in bold.