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. 2022 Jul 16;14(14):3458. doi: 10.3390/cancers14143458

Figure 4.

Figure 4

MACC1 manipulation in SW480 and SW620 cell lines leads to differential expression in clock and EMT-related genes (A) Analysis of MACC1 expression between HCT116 WT, SW480 WT and SW620 WT (n = 3, mean ± SEM). Gene expression analysis of core-clock genes ARNTL, PER2 and NR1D1 (B) as well as related genes in EMT, cell cycle, death and metastasis (C) in SW480 MACC1 overexpressing (OE) and SW620 MACC1 knockout (KO) cells, respectively. Samples were collected at 24 h after synchronization. Each condition was compared with its respective control cell line. (n = 3, mean ± SEM). (D,E) ARNTL-promoter activity in SW620 MACC1 knockout (D) and SW480 MACC1 overexpressing (E) cell lines compared with the respective control condition. Tcontrol = 24.2 ± 0.3 h and TMACC1 OE = 25.0 ± 0.1 h, mean ± SEM. Measurements were made over five days using live-cell bioluminescence readouts. Period, phase and amplitude were measured using Chronostar. (n = 3, mean ± SEM). * p < 0.05, ** p < 0.01, *** p < 0.001; two-tailed unpaired t-test.