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List and sequence of all primers designed in-house which were used for RT-qPCR analysis.
| Target Gene | Forward Primer (5′–> 3′) | Reverse Primer (5′–> 3′) |
|---|---|---|
| CD44 | ACACAAATGGCTGGTACGTCT | CCGTGGTGTGGTTGAAATGG |
| CD133 | CCCCAGGAAATTTGAGG AAC | TCCAACAATCCATTCCCTGT |
| ECAD | ATTGCAAATTCCTGCCATTC | CTCTTCTCCGCCTCCTTCTT |
| SIRT1 | AGGCCACGGATAGGTCCATA | GTGGAGGTATTGTTTCCGGC |
| MACC1 | TTCTTTTGATTCCTCCGGTGA | ACTCTGATGGGCATGTGCTG |
