Table 2.

Morphology, immunohistochemistry, and molecular analysis.

Case	Tumor Morphology	Necrosis	MF/50 HPFs	c-KIT	DOG-1	CD34	Desmin	Other IHCs	Molecular Analysis
1	spindle	Present	36	+	+	ND	-	SMA-, SOX10-	c-KIT A502_Y503 (insertion); RB1 c.1128-1G > C
2	epithelioid and spindle	Present	8	+	+	+ (weak)	ND	AE1/AE3-	ND
3	spindle	Absent	17	+	ND	+	-	ND	c-KIT exon 11 deletion/insertion mutation
4	epithelioid and spindle	Present	35	+	ND	+	-	Actin+ (weak), S100-	ND
5	epithelioid and spindle	Absent	6	+	ND	-	-	Actin-, SMA+ (weak), Cam5.2-, S100-, HMB45-, Melan A-, Inhibin-, calretinin-	ND
6	epithelioid and spindle	Absent	7	+	+	ND	ND	SMA-	c-KIT Exon 11 mutation (1732_1737dup: p.Y578_D579dup), additional mutations pV654A (exon 13) and L783V (exon 16); RB1 p.R355fs
7	spindle	Absent	3	+	ND	+	ND	ND	c-KIT c.1510insGCCTAT(p.504inAlaTyr) exon 9 mutation
8	spindle	Present	2	+	+	+	-	AE1/AE3-, HMB45-, ER-, PR-, CD68-, S100-	ND
9	epithelioid and spindle	Present	20	+	+	ND	-	SF-1-, Inhibin-, HMB45-, Cathepsin K+ (weak)	ND
10	epithelioid and spindle	Present	40	+	ND	+	-	Caldesmon+ (focal), SMA+ (weak), Actin-, ER-, PR-, HMB45-, S100-	ND
11	spindle	Present	2	+	+	ND	-	S100-	c-KIT p.W557R
12	spindle	Absent	6	+	ND	+	ND	AE1/AE3-, Cam5.2-, Calretinin-, Synaptophysin-, SMA-	c-KIT p.M552_V555del
13	spindle	Absent	38	+	+	ND	ND	ND	c-KIT p.A502_Y503dup and p.N822K
14	spindle	Absent	3	+	+	+	-	Actin-, S100-, CD10-, STAT6-, ALK-	ND
15	spindle	Absent	3	+	ND	+	-	SMA-, S100-	ND
16	epithelioid	Present	53	+	ND	+	-	AE1/AE3-, SMA-. S100-, Melan A-	ND
17	epithelioid and spindle	Present	33	+	ND	+	ND	ND	ND
18	epithelioid and spindle	Absent	2	+	+	ND	-	AE1/AE3-, Cam5.2-, CD10-, SF1-, Cyclin D1-, ER-, PR+ (weak), SDHB +	FGFR3 p.G145S
19	epithelioid	Absent	27	+	ND	ND	+ (weak)	AE1/AE3-, SMA-, S100-	ND
20	spindle	Present	25	+	+	ND	-	AE1/AE3-, HMB45-, Melan A-, Cathepsin K+	ND

HPF: High power field; IHC: Immunohistochemistry; MF: Mitotic figure; ND: Not done.