Table 5.
Type I isobolographic analysis of interactions for parallel concentration-response relationship lines between amantadine and MTO at the fixed drug concentration ratio of 1:1 in three melanoma cell lines, FM55P, FM55M2 and SK-MEL28. Results are median inhibitory concentrations (IC50 values in μg/mL ± S.E.M.) for two-drug mixtures, determined either experimentally (IC50exp) or theoretically calculated (IC50add) from the equations of additivity, blocking proliferation in 50% of tested cells in three melanoma cell lines (FM55M2, FM55P and SK-MEL28) measured in vitro by the MTT assay. * p < 0.05 vs. the respective IC50add value.
| Cell Line | IC50exp (μg/mL) | nexp | IC50add (μg/mL) | nadd | Interaction |
|---|---|---|---|---|---|
| FM55M2 | 14.39 ± 2.97 | 96 | 34.76 ± 9.01 * | 164 | Synergy |
| FM55P | 9.94 ± 1.96 | 96 | 20.18 ± 6.16 | 164 | Additivity |
| SK-MEL28 | 33.83 ± 5.80 | 96 | 35.79 ± 5.16 | 164 | Additivity |