Figure 2.

Targeted proteomic analysis of clinical stool specimens using the PRM assay. (A) Experimental workflows for protein extraction from a human stool sample. (B) The whole protein lysates obtained from clinical samples were separated using a 5–20% polyacrylamide gel, followed by detection using SYPRO Ruby staining. Each list of proteins detected by shotgun DDA-MS analysis is shown in Supplemental Table S2. Lane 1, Patient 1; Lane 2, Patient 2; Lane 3, Patient 3; Lane 4, Patient 4. (C) Example of PRM assay results for Patient 1 infected with the GII.3 VP1 genotypes of HuNoV.