. 2022 Jun 28;14(7):1416. doi: 10.3390/v14071416

Table 1.

Target peptides for a PRM assay of HuNoV VP1 protein.

Norovirus VP1	Peptide	Start-End	Charge	Light m/z	Heavy m/z	Fragment Ions Analyzed	LLOD * (fmol)	LLOQ ** (fmol)	ULOQ *** (pmol)	CV of Light Peptide in the Linearity Range (%)
GI.1_M87661/ Norwalk/68/US	TLDPIEVPLEDVR	154–166	2	748.404	753.408	y10⁺, y8⁺, y6⁺, y2⁺	<0.19	780	>100	23.19
	TGGGTGDSFVVAGR	192–205	2	640.815	645.819	y9⁺, y7⁺, y4⁺, y3⁺	<0.19	3.05	25	23.92
	LVGTTPVSLSHVAK	276–289	3	470.277	472.948	y9⁺, y8⁺, y7⁺, y2⁺	<0.19	6.1	12.5	23.09
GII.2_X81879/ Melksham	VFGVISQR	332–339	2	453.264	458.268	y6⁺, y4⁺, y3⁺, b3⁺	<0.19	3.05	12.5	23.10
GII.2_X81879/ Melksham	YAGALNLNTNLAPSVAPVFPGER	410–432	2	1186.124	1191.128	y12⁺, y11⁺, y7⁺, y4⁺	<0.19	780	12.5	6.97
GII.3_U02030/ Tronto	VFGVASQR	343–340	2	432.240	437.244	y6⁺, y4⁺, b2⁺, b3⁺	<0.19	3.05	3.13	23.15
	SQLPSSGGR	443–451	2	444.730	449.734	y7⁺, y6⁺, y2⁺, b2⁺	N.D.	N.D.	N.D.	N.D.
	NGDSPITVPPNGYFR	510–524	2	817.402	822.406	y11⁺, y9⁺, y7⁺, b2⁺	<0.19	1.53	50	19.38
GII.4_X76716/ Bristol	NNFYHYNQANDSTLK	163–177	3	610.280	612.951	y7⁺, y6⁺, y4⁺, b2⁺	<0.19	6.1	6.25	23.05
GII.4_X76716/ Bristol	ANNAGDDVFTVSCR	188–201	2	763.339	768.343	y10⁺, y6⁺, y3⁺, b2⁺	<0.19	6.1	>100	21.92
GII.6_AJ277620/ Seacroft/90/UK	GTLISQTAR	287–295	2	473.769	478.774	y7⁺, y6⁺, y5⁺, y1⁺	<0.19	1.53	6.25	15.95
	NHPLHVQVK	307–315	3	357.873	360.545	y5⁺, y4⁺, y1⁺, b2⁺	N.D.	N.D.	N.D.	N.D.
	LGTILIK	380–386	2	379.263	383.270	y2⁺, y1⁺, b3⁺, b2²⁺	<0.19	3.05	0.39	19.53
GII.17_AY502009/ CS-E1/2002/US	NVGSNPNTTR	340–349	2	530.263	535.267	y8⁺, y5⁺, y1⁺, b2⁺	N.D.	N.D.	N.D.	N.D.
GII.17_AY502009/ CS-E1/2002/US	FTPVGIK	387–393	2	381.231	385.239	y5⁺, y3⁺, y1⁺, b2⁺	<0.19	6.1	12.5	19.65

*, Lower limit of detection; **, Lower limit of quantification; ***, Upper limit of quantification; N.D., not determined.