Table 2.
Detection of VP1 genotype in human stool specimens using the PRM assay.
| Patient No. | PCR Analysis | Extracted Total Protein (μg) | PRM Assay | ||
|---|---|---|---|---|---|
| Genotype | Ct Value | Detected Target Peptide | Concentration (fmol/μg) | ||
| 1 | GII.3 | 19 | 693 | GII.3 NGDSPITVPPNGYFR_2+ | 13.17 |
| GII.3 VFGVASQR_2+ | <LLOQ * | ||||
| 2 | GII.4 | 19 | 319 | GII.4 ANNAGDDVFTVSCR_2+ | <LLOQ * |
| 3 | GII.P16/GII.2 | 18 | 545 | GII.2 YAGALNLNTNLAPSVAPVFPGER_2+ | 5.99 |
| 4 | GII.P16/GII.2 | 19 | 101 | GII.2 YAGALNLNTNLAPSVAPVFPGER_2+ | <LLOQ * |
*, Less than the lower limit of quantification.