Figure 1.

Experimental Overview. F0 zebrafish were dietarily exposed from 17 to 59 days post fertilization (dpf) to diets contaminated with (left to right) 0 (control), 1, 10, or 100 ng/g PFHxA. Immediately following dietary exposure, measurements of morphometrics and body burden were performed. Following a subsequent depuration period, fecundity and adult behavior were assessed after 90 dpf. One spawning event was used to generate the F1 generation, which underwent assessments of developmental toxicity, larval and juvenile behavior, fecundity, and adult behavior. The same experimental protocols were used to interrogate transgenerational effects in the F2 generation.