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Differential gene expression in forkhead mutants. foxl1-/- (A), foxc1a-/- (C), and foxc1b-/- (D) embryos at 6 dpf using TaqMan RT-qPCR to confirm genes of interest from RNA-Seq screen. Targets of interest were examined at 10 dpf in foxl1 mutants (B), demonstrating a recovery in gene expression. Fold change between WT and foxl1-/- plotted with WT values set to 1 (* p < 0.05, ** p < 0.01, *** p < 0.001).
