TABLE 2.

K_m and TN_max values for three catalytic systems^a

Catalyst	Concn	Km (mM) for H2O2	TNmax (s−1)
Horse radish peroxidase	1 nM heme or protein	0.74	2,220
T. fusca protein	30 nM protein	18b	0.102c
CuSO4	5 μM Cu(II)	22b	0.007c

^aKinetic constants K_m and TN_max are from the data in Fig. 6. Conditions were as described in the legend to Fig. 6 and Materials and Methods. 

^bK_mvalues for H₂O₂ for T. fusca pseudoperoxidase and free cupric ions are similar. 

^cTurnover for the T. fusca protein is ∼14 times that of free Cu, but the protein may be associated with up to 20 mol of Cu/mol of protein (17).