Table 1.
The number of mismatches in the primers/probes of the Pan-RSV and duplex assays using registered sequences.
| Year Registered | All | 2010–2015 | 2016–2022 | ||
|---|---|---|---|---|---|
| Total number | 2935 | 1069 | 1185 | ||
| Pan-RSV | Forward | Number of mismatched sequences | 60 (2.0%) | 4 (0.37%) | 6 (0.5%) |
| (Within 10 nucleotides from the 3′-end) | 57 (1.9%) | 3 (0.28%) | 6 (0.5%) | ||
| Reverse | Number of mismatched sequences | 1457 (49.7%) | 456 (42.7%) | 703 (59.3%) | |
| (Within 10 nucleotides from the 3′-end) | 275 (9.4%) | 10 (0.9%) | 257 (21.7%) | ||
| Probe | Number of mismatched sequences | 1579 (53.8%) | 623 (58.3%) | 544 (45.9%) | |
| Duplex | Forward | Number of mismatched sequences | N.D. * | N.D. | 25 (2.1%) |
| (Within 10 nucleotides from the 3′-end) | N.D. | N.D. | 18 (1.5%) | ||
| Reverse | Number of mismatched sequences | N.D. | N.D. | 34 (2.9%) | |
| (Within 10 nucleotides from the 3′-end) | N.D. | N.D. | 3 (0.3%) |
* N.D., not determined.