TABLE 1.
Distribution of 119 zucchini purée isolates according to REP type and identities of representative strainsa
| REP typeb with primer group:
|
No. of isolates | Colonies on:
|
Representative straine | API 50CHB/20E profile
|
16S ribosomal DNA sequence analysis
|
||||
|---|---|---|---|---|---|---|---|---|---|
| REP | REP-Dt | MYP agar c | J-agard | Taxon | Identity (%) | Species closely related | Identity (%) | ||
| P1 | 1 | − | II5 | P14-7 | B. circulans group 2 | 91.2 | P. azotofixans | 96 | |
| P2 | 1 | − | II5 | P22-9 | B. circulans group 2 | 98.8 | P. azotofixans | 96 | |
| C | 9 | − | II3 | P52-3 | B. circulans group 2/P. macerans | 83/16 | P. amylolyticus | 99 | |
| A | 4 | L+ | III | P12-9 | P. polymyxa | 99.5 | P. polymyxa | 99 | |
| B | 21 | L+ | II2 | P51-3 | P. polymyxa | 99.9 | P. polymyxa | 96 | |
| L | 1 | − | II4 | P15-9 | B. lentus | 99.9 | P. ilinoisensis | 90 | |
| Id | E1 | 5 | − | IV3 | P53-2 | Brevibacillus brevis/B. sphaericus | 65/35 | B. macroides/B. maroccanus | 98 |
| E2 | 2 | − | IV1 | P51-5 | Brevibacillus brevis | 97.3 | B. macroides/B. maroccanus | 99 | |
| F | 1 | − | IV2 | P54-2 | Brevibacillus brevis/B. sphaericus | 34/65 | B. macroides/B. maroccanus | 99 | |
| I | 1 | − | II6 | P22-5 | B. circulans group 1/B. macerans | 53/23 | B. circulans | 99 | |
| R | 2 | − | II6 | P22-10 | B. circulans group 1 | 99.7 | B. circulans | 99 | |
| D | 1 | − | VII | P53-6 | Brevibacillus brevis | 99.9 | B. sphaericus | 94 | |
| G | 13 | − | V1, V2, V3 | P22-8 | B. licheniformis/B. subtilis | 54/46 | B. licheniformis | 99 | |
| K1 | 1 | − | V2 | P23-12 | B. licheniformis | 99.9 | B. licheniformis | 99 | |
| K2 | 2 | − | V4 | P22-11 | B. licheniformis | 98.2 | B. licheniformis | 99 | |
| K3 | 2 | − | V1 | P23-17 | B. licheniformis | 99.9 | B. licheniformis | 98 | |
| H1 | 1 | − | V12 | P22-17 | B. licheniformis/B. subtilis | 80/20 | B. subtilis | 99 | |
| H2 | 3 | − | V11 | P23-5 | B. amyloliquefaciens/B. subtilis | 95/5 | B. subtilis | 99 | |
| Id | J | 1 | − | I3 | P23-18 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | M1 | 6 | − | I1, I2 | P15-7 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | M2 | 2 | − | I2 | P15-4′ | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | M3 | 1 | − | I1 | P13-7 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | M4 | 2 | − | I1 | P13-5 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | M5 | 1 | − | I1 | P14-2′ | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | M6 | 6 | − | I1, I2 | P12-10 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | N | 9 | − | I1, I2 | P12-4 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | 01 | 1 | − | I1 | P14-4 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | O2 | 3 | − | I1, I2 | P14-5 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | Q1 | 1 | − | I3 | P23-2 | B. pumilus | 99.9 | B. pumilus | 98 |
| Id | Q2 | 1 | − | I2 | P11-7 | B. pumilus | 99.9 | B. pumilus | 98 |
| Id | S | 2 | − | I3 | P23-3 | B. pumilus | 99.9 | B. pumilus | 99 |
| Id | T | 4 | + | III | P22-3 | B. mycoides | 98.8 | B. cereus group | 99 |
| Id | U | 1 | + | III | P22-4 | B. mycoides/B. cereus | 98/1 | B. cereus group | 99 |
| Id | V | 1 | + | III | P15-3 | B. cereus group 1/B. mycoides | 52/48 | B. cereus group | 99 |
| Id | W | 6 | + | III | P13-4 | B. cereus group 1 | 99.5 | B. cereus group | 99 |
Results for which the AP150CHB/20E system was in disagreement with rrs gene sequence identity are in boldface.
Letters represent major different REP types; numbers following letters represent related subgroups differentiated by one or two minor bands on electrophoretic patterns; Id, low discriminative electrophoretic pattern (fewer than four bands).
+, lecithinase positive and manitol negative; L+, lecithinase positive only; −, lecithinase negative and manitol negative.
I to VII, major distinct morphotypes; numbers following morphotypes represent subgroups according to mucosal aspect (types I, IV, V, and VI) or according to the colony size and thickness (type II).
Strain randomly selected from each REP type for API50CHB/20E identification and rrs gene sequencing.