Table 1.
Primers and probes designed for the multiplex real time RT-PCR assays detecting the various aMPV subtypes. Primers and probes were designed using reference sequences (a subtype A Acc. Num. MF093139.1; b subtype B Acc. Num MN729604.1; c subtype C Acc. Num. AY579780.1; d subtype detected in parakeets Acc. Num. MK491499.1; e subtype detected in gulls Acc. Num. MN175553.1).
| Primer/Probe | Sequence 5′→3′ | Position |
|---|---|---|
| aMPV A Forward | CACCCAGGAGCAGCCAACTA | 6333–6352 a |
| aMPV A Probe | 5′HEX TGCTGGAGTCGCACTTGGTGC 3′BHQ1 | 6355–6375 a |
| aMPV A Reverse | TGTTCGAGCCGTTTGTAATCCTC | 6386–6408 a |
| aMPV B Forward | TGGGCAGAAAATGGATCCTTACA | 6209–6231 b |
| aMPV B Probe | 5′FAM GGCGACTGGAGCAGGAAAGTTTGA 3′BHQ1 | 6301–6324 b |
| aMPV B Reverse | CCATCAACAACTTGCACATACCC | 6332–6354 b |
| aMPV C Forward | CAAGGGATCCAGAGGTGAGG | 6427–6446 c |
| aMPV C Probe | 5′TAMRA CAAGCCCCAGGCCAATGAAG 3′BHQ2 | 6461–6480 c |
| aMPV C Reverse | GAGGTTCCTGCTTGGGTTTG | 6487–6506 c |
| aMPV PAR-05 Forward | GCGAAACCGATCCAAGACTC | 6543–6562 d |
| aMPV PAR-05 Probe | 5′CY5 CACACAAGCAGACCACAACAACAGA 3′BHQ3 | 6595–6619 d |
| aMPV PAR-05 Reverse | GAATCTTTGGGGCTTGCTTG | 6629–6648 d |
| aMPV GuMPV B29 Forward | AAGTTGCGGAGTCAGTGCAA | 12240–12259 e |
| aMPV GuMPV B29 Probe | 5′FAM CAGGGAGGAGCCCTCGTCAA 3′BHQ1 | 12281–12300 e |
| aMPV GuMPV B29 Reverse | CGGTGGCACTATGTCGATGT | 12326–12345 e |