Omegaven® reduces the barrier formation at high concentrations. Omegaven® and Intralipid® lipid emulsions at 0.01, 0.1 and 1 mM concentrations were added to endothelial cells during cell seeding on the ECIS electrode (A), during the logarithmic growth phase (B), and in cells of the plateau growth phase (C). The barrier formation was monitored by an ECIS®Zθ (Z Theta) device, and 60 measurements of resistance (ohm) in 70 h were recorded for cells grown in triplicates. In addition, areas under the curve (D) for the graph presented in (A–C) were analyzed using (GraphPad, San Diego, CA, USA), X.Y. analysis/Area under curve function.