Figure 5.

Expression of MHCI, MHCII, and co-stimulatory molecules CD40, CD86 on the surface of the dendritic cells induced with adjuvanted gonococci vaccine microparticles. DC 2.4 cells (5 × 10⁴ cells per well) were induced with Gc-MP (100 µg/well) alone or combined with adjuvants (Alum MP or AddaVax™ MP) or inactivated or fixed whole-cell gonococci in suspension (100 µg/well) then incubated at 37 °C for 24 h. The surface expression of MHCI (A), MHCII (B), CD86 (C) and CD40 (D) on the DCs induced by adjuvated Gc-MP was compared to unstimulated DC 2.4 control cells. All data were repeated as three independent experiments with three replicates in each experiment. Data are expressed as mean ± SD, One-way ANOVA-Kruskal-Wallis test. * p < 0.05 significant, ** p < 0.01 very significant.