Table 1.
Summary of Lolium multiflorum single-molecule real-time sequencing results.
| CK | DR | Total | |||
|---|---|---|---|---|---|
| Subreads base (G) | 15.48 | 15.24 | 30.72 | ||
| number | 6,944,546 | 6,843,321 | 13,787,867 | ||
| Average length (bp) | 2229 | 2227 | 2228 | ||
| N50 (bp) | 2652 | 2694 | - | ||
| CCS | 488,868 | 538,115 | 1,026,983 | ||
| 5′-primer | 456,294 | 489,188 | 945,482 | ||
| 3′-primer | 462,375 | 501,744 | 964,119 | ||
| Poly-A | 458,539 | 497,870 | 956,409 | ||
| Full length | 424,911 | 454,129 | 879,040 | ||
| FLNC | 403,543 | 431,325 | 834,868 | ||
| Before Correction | After Correction | Before Correction | After Correction | After Correction | |
| Total nucleotides | 469,861,260 | 471,334,422 | 519,052,371 | 520,093,777 | 991,428,199 |
| Total number | 184,267 | 184,267 | 201,378 | 201,378 | 385,645 |
| Mean length (bp) | 2550 | 2558 | 2578 | 2583 | 2571 |
| Min length (bp) | 192 | 193 | 200 | 197 | 193 |
| Max length (bp) | 14,449 | 14,437 | 14,422 | 14,348 | 14,437 |
| N50 (bp) | 2755 | 2759 | 2795 | 2798 | - |
| N90 (bp) | 1774 | 1778 | 1782 | 1784 | - |
| Transcripts Length Interval | Number of Transcripts | Number of Unigenes | Number of Transcripts | Number of Unigenes | |
| <500 bp | 1973 | 1086 | 418 | 211 | |
| 500–1k bp | 4402 | 3289 | 3408 | 2299 | |
| 1–2k bp | 46,268 | 17,602 | 50,843 | 25,263 | |
| 2–3k bp | 80,106 | 40,389 | 87,946 | 45,276 | |
| >3k bp | 51,518 | 32,554 | 58,763 | 39,986 | |
| Total | 184,267 | 94,920 | 201,378 | 113,035 | |