Figure 6.

Cisplatin, paclitaxel, docetaxel (used at 25 ng/mL) and curcumin (used at 6.9 µg/mL–18.73 µM) reduce the size of DU145 cells derived organoids. (A): Three days after cells embedding in Matrigel, representative images were acquired as described in Method section. The 3D cultures were left untreated or treated in the absence or presence of the indicated drugs for 12 days. Were shown phase-contrast images captured on 12th day. Scale bar, 100 µ. (B): In the graph is represented the analysis of organoid size from DU145 cells untreated or treated with the drugs reported in the figure for 12 days. The area of organoids was calculated using Leica suite software. Three different experiments, each in duplicate, were performed and results were expressed as fold increase over the basal level analyzed after 3 days. Means and standard error of the means (SEMs) are shown, and “n” represents the number of the experiments. *: p < 0.05 for the indicated experimental points vs. the untreated controls.