Figure 7.

Cisplatin (used at 3.32 µg/mL–11.03 µM), paclitaxel (used at 15.6 µg/mL–18.27 µM), docetaxel (used at 20 µg/mL–24.76 µM) and curcumin (used at 6.9 µg/mL–18.73 µM) reduce the size of PC3 cells derived organoids. (A): Three days after cells embedding in Matrigel, representative images were acquired as described in Methods. The 3D cultures were left untreated or treated in the absence or presence of the indicated drugs for 10 days. Were shown phase-contrast images captured on 10th day. Scale bar, 100 µ. (B): In the graph is represented the analysis of organoid size from PC3 cells untreated or treated with the drugs reported in the figure for 10 days. The area of organoids was calculated using Leica suite software. Three different experiments, each in triplicate, were performed and results were expressed as fold increase over the basal level analyzed after 3 days. Means and standard error of the means (SEMs) are shown, and “n” represents the number of the experiments. *: p < 0.05 for the indicated experimental points vs. the untreated controls.