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. 2022 Jul 9;14(14):3348. doi: 10.3390/cancers14143348

Figure 9.

Figure 9

Cisplatin (used at 3.32 µg/mL–11.03 µM), paclitaxel (used at 15.6 µg/mL–18.27 µM), docetaxel (used at 20 µg/mL–24.76 µM) and curcumin (used at 6.9 µg/mL–18.73 µM) induce apoptosis in PC3 cells. (A): Phase-contrast and in situ cell death KIT-stained images (green) of organoids left untreated or treated in the absence or presence of the indicated drugs for 15 days are shown. Scale bar, 100 µ. (B): Phase-contrast and Ki-67-stained images (red) of organoids untreated for 15 days are shown. Scale bar, 100 µ. In (A,B), images are representative of three different experiments, each in duplicate. (C): Cell viability was evaluated by MTT assay in PC3 organoids after 10 days of culture. The graph in (C), represents the organoids’ cell viability expressed as % of control, assumed as 100% of viability. Four independent experiments were carried out. Means and standard error of the means (SEMs) are shown, and “n” represents the number of the experiments. *: p < 0.05 for the indicated experimental points vs. the untreated controls.