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. 2022 Jul 9;14(14):3344. doi: 10.3390/cancers14143344

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© 2022 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mixing mouse MC3T3÷E1 osteoblasts with human stroma at a ratio of 1:10 increases the capacity to support dormant MCF÷7 cell clones almost two-fold. FGF÷2 produced by stoma is sufficient to support dormancy [79], with exogenous FGF÷2 having no additional effect on stroma or mixing experiments (p > 0.05). However, exogenous FGF÷2 is necessary for dormancy on MC3T3÷E1 monolayers, suggesting they do not produce and export FGF-2. The dormancy support efficiency on MC3T3÷E1 cells is greater than that of stroma by more than two-fold (p < 0.05). Error bars ±S.D.