Table 1.
Summary of the oligonucleotide primers used in this study.
| Virus and Primer | Sequence (5′ to 3′) | Product Size (bp) |
|---|---|---|
| Canine distemper virus a | ||
| CDV-F | AGCTAGTTTCATCTTAACTATCAAATT | 83 |
| CDV-R | TTAACTCTCCAGAAAACTCATGC | |
| Canine respiratory coronavirus b | ||
| BCoV-F | CTGGAAGTTGGTGGAGTT | 85 |
| BCoV-R | ATTATCGGCCTAACATACATC | |
| Canine pneumovirus c | ||
| CnPnV-For | AAGATAAATTCTTCTATGAAAACAGAATGA | 110 |
| CnPnV-Rev | CCCATCGTAAGTGAGGTTTCTATT | |
| Canid herpesvirus 1 d | ||
| CHV-For | ACAGAGTTGATTGATAGAAGAGGTATG | 136 |
| CHV-Rev | CTGGTGTATTAAACTTTGAAGGCTTTA | |
| Canine influenza virus e | ||
| M-Flu1 | CTTCTAACCGAGGTCGAAACGTA | 147 |
| M-Flu2 | GGATTGGTCTTGTCTTTAGCCA | |
| Bordetella bronchiseptica f | ||
| FLA 2 | AGGCTCCCAAGAGAGAAAGGCTT | 237 |
| FLA 4 | TGGCGCCTGCCCTATC | |
|
Streptococcus equi subsp. zooepidemicusg |
||
| sodA equi/zooep-F | CAGCATTCCTGCTGACATTCGTCAGG | 235 |
| sodA equi/zooep-R | CTGACCAGCCTTATTCACAACCAGCC | |
| Canine adenovirus h | ||
| CAV-F | AGTAATGGAAACCTAGGGG | 80 |
| CAV-R | TCTGTGTTTCTGTCTTGC | |
| Mycoplasma cynos i | ||
| Myc1-For | CACCGCCCGTCACACCA | 227 |
| M. cynos-Rev | GATACATAAACACAACATTATAATATTG | |
| Mycoplasma canis i | ||
| Myc1-For | CACCGCCCGTCACACCA | 247 |
| M. canis-Rev | CTGTCGGGGTTATCTCGAC |
a Oligonucleotide positions that refer to the sequence of canine distemper virus strain Onderstepoort. b Oligonucleotide positions that refer to the sequence of BCoV strain Mebus (GenBank accession no. U00735). c Oligonucleotide positions that refer to the sequence of CnPnV strain dog/Ane4/USA/2008 (GenBank accession no. HQ734815). d Oligonucleotide positions that refer to the sequence of CHV-1 glycoprotein B (GenBank accession no. AF361073). e Primers and probe were designed based on the sequence homology of the matrix gene (segment 7) among different influenza A virus isolates, belonging to subtypes from H1–H13 isolated in avian, human, swine and equine hosts. f An upstream sequence of the flagellin gene (fla gene), including the sigma consensus site and putative ribosome-binding site, was used as a target DNA region. g Oligonucleotide positions that refer to the sequences of S. equi subsp. zooepidemicus sodA gene (GenBank accession no. Z95902) encoding superoxide dismutase A. h Oligonucleotide positions that refer to the sequences of CAdV-1 (GenBank accession no. AC000003) and CAdV-2 (GenBank accession no. AC000020) within the highly conserved hexon gene. i Regarding Mycoplasma spp., primers were designed based on the sequence of 16S/23S rRNA intergenic spacer (IGS) species-specific regions, which are as follows: M. spumans, AF538684; M. opalescens, AF443612; Mycoplasma sp. strain VJC 358, AY246564; M. gateae, AF443609; M. cynos, AF538682; M. molare, AF443611; M. molare, AF538683; M. maculosum, AF443610; M. opalescens, AF538961; Mycoplasma sp. strain HRC 689, AF527624; Mycoplasma arginini, AF443604; M. cynos, AF443606; M. felis, AF443608; M. edwardii, AF443607; M. canis, AF443605.